Studies of the Biosynthesis of Thyroxine. I. Purification and Properties of a Particulate Iodide Peroxidase from Thyroid Tissue.
نویسندگان
چکیده
Previously, a number of workers have described preparations of thyroid tissue which catalyze the iodination of free or proteinbound tyrosine (l-7). It was demonstrated that the enzymatic activity was mitochondrial in location, and depending upon the method of preparation or assay, various metals and cofactors were implicated in the reaction. Serif and Kirkwood (1, 2) found that addition of certain metals increased activity, whereas Chaikoff, Taurog, Potter, and Tong (3, 4) reported that flavin mononucleotide and manganese ions stimulated enzymatic catalysis. Alexander (5) suggested that the oxidation of iodide is a peroxidation and found that the addition of hematin to preparations of thyroid tissue stimulated activity (6). In each of these studies, the activity of the enzymes was followed by measuring the production of iodinated tyrosines with radioactive iodine. The present paper describes the purification and properties of a highly active particulate enzyme system isolated from beef thyroid tissue which catalyzes the peroxidation of iodide to iodine.
منابع مشابه
Studies of the Biosynthesis of Thyroxine I. PURIFICATIOIS AND PROPERTIES OF A PARTICULATE IODIDE PEROXIDASE FROM THYROID TISSUE*
Previously, a number of workers have described preparations of thyroid tissue which catalyze the iodination of free or proteinbound tyrosine (l-7). It was demonstrated that the enzymatic activity was mitochondrial in location, and depending upon the method of preparation or assay, various metals and cofactors were implicated in the reaction. Serif and Kirkwood (1, 2) found that addition of cert...
متن کاملAutoimmune Thyroid Disorder in Children and Adolescents with Type I Diabetes Mellitus
Background Type one diabetes mellitus (Type 1 DM) is the most common type of diabetes in children and adolescents, arising through a complex interaction of immune, genetic and environmental factors. Autoimmune thyroid disease is the most frequent disorder associated with Type one diabetes mellitus. This study aimed to evaluate incidence of autoimmune thyroid disease in children and adolescents ...
متن کاملThe structural specificity of sulfanilamide-like compounds as inhibitors of the invitro conversion of inorganic iodide to thyroxine and diiodotyrosine by thyroid tissue.
By means of radioactive iodine it was demonstrated in this laboratory that surviving slices of thyroid tissue can incorporate inorganic iodide into diiodotyrosine and thyroxine (1). When 300 mg. of thyroid slices were incubated for 2 hours in a Ringer’s medium containing radioactive inorganic iodide, as much as 60 per cent of the labeled iodine was organically bound, about 50 per cent as diiodo...
متن کاملEffect of Thyrotoxicosis on Gene Expression of Hydrogen Sulfide-producing Enzymes in Epididymal Adipose Tissue of Male Rats
Introduction: Thyroid hormones are involved in the regulation of hydrogen sulfide (H2S) biosynthesis. The aim of this study is to determine effects of thyrotoxicosis on H2S levels and mRNA expression of cystathionine-β-synthase (CBS), cystathionine-γ-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (3-MST) in the adipose tissue of rat. Materials and Methods: Male rats were divided into the ...
متن کاملThe sodium iodide symporter and thyroid disease.
The movement of a variety of species from the iodine-rich environment of the sea to the relatively iodine-deficient land has necessitated the development of mechanisms which will trap more efficiently inorganic iodide (I – ) necessary for the formation of thyroid hormones (Venturi et al. , 2000). In man as in other mammals iodide uptake by the thyroid is mediated via a TSHdependent transmembran...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 239 شماره
صفحات -
تاریخ انتشار 1964